Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | - |
Pseudomonas straminea | |
Monoiodoacetic acid | weaker inhibitor than p-chloromercuribenzoate or HgCl2 | Pseudomonas straminea | |
additional information | not: various metal ions, metal chelating reagents, reducing reagents | Pseudomonas straminea | |
N-ethylmaleimide | weaker inhibitor than p-chloromercuribenzoate or HgCl2 | Pseudomonas straminea | |
p-chloromercuribenzoate | - |
Pseudomonas straminea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0175 | - |
NADP+ | - |
Pseudomonas straminea | |
0.02 | - |
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde | in the presence of 0.110 mM NADP+ | Pseudomonas straminea | |
0.056 | - |
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde | in the presence of 0.110 mM NAD+ | Pseudomonas straminea | |
0.284 | - |
NAD+ | - |
Pseudomonas straminea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Pseudomonas straminea |
67000 | - |
gel filtration | Pseudomonas straminea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas straminea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using gel filtration on Sephadex G-200 | Pseudomonas straminea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.413 | - |
- |
Pseudomonas straminea |
Storage Stability | Organism |
---|---|
-20°C, for at least 4 months | Pseudomonas straminea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NAD+ | - |
Pseudomonas straminea | 4-carboxy-2-hydroxy-cis,cis-muconate + NADH | - |
? | |
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+ | or NAD+ | Pseudomonas straminea | 4-carboxy-2-hydroxy-cis,cis-muconate + NADPH | - |
? | |
additional information | not: unsubstituted aliphatic or aromatic aldehydes | Pseudomonas straminea | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, SDS-PAGE | Pseudomonas straminea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
assay at | Pseudomonas straminea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
10 min, pH 7.0, 50% loss of activity | Pseudomonas straminea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Pseudomonas straminea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.3 | at pH 5.5 and 9.3: about 50% of activity maximum | Pseudomonas straminea |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | highest stability | Pseudomonas straminea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | can replace NADP+, but with lower affinity | Pseudomonas straminea | |
NADP+ | - |
Pseudomonas straminea |